Sphingobacterium kyonggiense sp. nov., isolated from chloroethene-contaminated soil, and emended descriptions of Sphingobacterium daejeonense and Sphingobacterium mizutaii

Abstract

A Gram-reaction-negative, strictly aerobic, rod-shaped, non-motile strain, designated 2-1-2T, was isolated from perchloroethylene/trichloroethene-contaminated soil in Suwon, South Korea. A polyphasic approach was used to study the taxonomic position of strain 2-1-2T. Strain 2-1-2Tshowed highest 16S rRNA gene sequence similarities toSphingobacterium daejeonenseTR6-04T(97.9 %) andSphingobacterium mizutaiiATCC 33299T(97.1 %); sequence similarities to otherSphingobacteriumspecies were less than 93.0 %. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 2-1-2Tbelonged to the clade formed by members of the genusSphingobacteriumin the familySphingobacteriaceae. The G+C content of the genomic DNA was 36.6 mol%. Strain 2-1-2Tshowed the typical chemotaxonomic features of the genusSphingobacterium, with the presence of a ceramide phosphorylethanolamine (CerPE-2) as the major ceramide, menaquinone 7 (MK-7) as the predominant respiratory quinone and iso-C15 : 0, iso-C17 : 03-OH and summed feature 3 (comprising iso-C15 : 02-OH and/or C16 : 1ω7c) as the major fatty acids. On the basis of phylogenetic inference, fatty acid profile and other phenotypic properties, and DNA–DNA relatedness, strain 2-1-2Trepresents a novel species of the genusSphingobacterium, for which the nameSphingobacterium kyonggiensesp. nov. is proposed; the type strain is 2-1-2T( = KEMC 2241-005T = JCM 16704T). Emended descriptions ofSphingobacterium daejeonenseandSphingobacterium mizutaiiare also proposed.