Affiliation:
1. Department of Biology, Faculty of Art and Science, Ondokuz Mayis University, 55139 Kurupelit-Samsun, Turkey
2. German Collection of Microorganisms and Cell Cultures GmbH, 38124 Braunschweig, Germany
Abstract
A novel actinomycete, designated strain KT2025T, was isolated from arid soil collected from Magusa, northern Cyprus. The taxonomic position of the novel strain was established by using a polyphasic approach. The organism had chemical and morphological features consistent with its classification in the genus
Amycolatopsis
. Phylogenetic analyses based on 16S rRNA gene sequences supported the classification of the isolate in the genus
Amycolatopsis
and showed that the organism formed a cluster with
Amycolatopsis nigrescens
CSC17-Ta-90T,
Amycolatopsis minnesotensis
32U-2T,
Amycolatopsis sacchari
DSM 44468T and
Amycolatopsis dongchuanensis
YIM 75904T. 16S rRNA gene sequence similarity analysis indicated that strain KT2025T was most closely related to
Amycolatopsis lurida
DSM 43134T (97.5 %),
Amycolatopsis keratiniphila subsp. keratiniphila
DSM 44409T (97.4 %),
Amycolatopsis keratiniphila subsp. nogabecina
DSM 44586T (97.1 %),
Amycolatopsis nigrescens
DSM 44992T (97.1 %),
Amycolatopsis azurea
DSM 43854T (97.1 %) and
Amycolatopsis minnesotensis
DSM 44988T (96.9 %). The organism was found to have chemical features typical of members of the genus
Amycolatopsis
such as meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, and arabinose and galactose as diagnostic sugars. The predominant menaquinone was MK-9(H4). The polar lipids detected were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and hydroxy-phosphatidylethanolamine. The major fatty acids were iso-C16 : 0, iso-C15 : 0 and iso-C14 : 0. The G+C content of the genomic DNA was 70.8 mol%. Phenotypic data clearly distinguished the isolate from its closest relatives. The combined genotypic and phenotypic data and low levels of DNA–DNA relatedness with its closest relatives indicated that strain KT2025T represents a novel species of the genus
Amycolatopsis
, for which the name Amycolatopsis magusensis sp. nov. is proposed. The type strain is KT2025T ( = DSM 45510T = KCTC 29056T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
14 articles.
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