Rhodanobacter ginsengisoli sp. nov. and Rhodanobacter terrae sp. nov., isolated from soil cultivated with Korean ginseng

Abstract

Two bacterial isolates from ginseng fields in Korea, strains GR17-7T and GP18-1T, were characterized using a polyphasic approach. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the Gammaproteobacteria, and showed that the closest phylogenetic relationships were with members of the genus Rhodanobacter. The 16S rRNA gene sequence similarity between strains GR17-7T and GP18-1T was 97.2 %. Both strains showed 16S rRNA gene sequence similarities of 95.2–96.9 % to type strains of recognized Rhodanobacter species. The G+C contents of the DNA of strains GR17-7T and GP18-1T were 61.0 and 62.5 mol%, respectively. According to the DNA–DNA hydridization tests, the hybridization value between strains GR17-7T and GP18-1T was 34 %. Strains GR17-7T and GP18-1T showed less than 32 % DNA–DNA relatedness with Rhodanobacter fulvus KCTC 12098T and Rhodanobacter spathiphylli LMG 23181T. Strains GR17-7T and GP18-1T were aerobic, Gram-negative, rod-shaped, and catalase- and oxidase-positive. Major fatty acids of both strains were iso-C17 : 1 ω9c and iso-C16 : 0. Based on the data presented, two novel Rhodanobacter species are proposed, with the names Rhodanobacter ginsengisoli sp. nov. (type strain GR17-7T=KACC 11762T=DSM 18993T) and Rhodanobacter terrae sp. nov. (type strain GP18-1T=KACC 11761T=DSM 19241T).