Rhodanobacter spathiphylli sp. nov., a gammaproteobacterium isolated from the roots of Spathiphyllum plants grown in a compost-amended potting mix

Author:

De Clercq Deborah1,Van Trappen Stefanie2,Cleenwerck Ilse2,Ceustermans An1,Swings Jean2,Coosemans Jozef1,Ryckeboer Jaak1

Affiliation:

1. Laboratory of Phytopathology and Plant Protection, Katholieke Universiteit Leuven, W. De Croylaan 42, B-3001 Leuven, Belgium

2. BCCM/LMG Bacteria Collection, Laboratory of Microbiology, University of Ghent, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium

Abstract

Three Gram-negative, yellow-pigmented strains were isolated from the rhizospheres of Spathiphyllum plants grown in a compost-amended potting mix. The strains showed biological control activity towards the root-rot plant pathogen Cylindrocladium spathiphylli, and were characterized to determine their taxonomic position. Cells of the strains were non-motile rods, and the strains were oxidase- and catalase-positive and unable to ferment most sugars tested. The three strains showed differences in growth temperature range, optimal growth temperature and some biochemical reactions. The majority of the fatty acids were branched, and large amounts of 15 : 0 iso and 17 : 1 iso ω9c were present. The 16S rRNA gene sequence (1497 bp) of strain B39T showed the highest level of similarity (98.5 %) to that of Rhodanobacter fulvus IAM 15025T, followed by Rhodanobacter lindaniclasticus LMG 18385T (96.0 %; strain no longer extant), Dyella koreensis CCUG 50883T (96.4 %), Dyella japonica DSM 16301T (96.3 %), Frateuria aurantia LMG 1558T (96.2 %) and Fulvimonas soli LMG 19981T (95.9 %). Less than 90 % 16S rRNA gene sequence similarity was observed for other members of the Gammaproteobacteria. The mean DNA–DNA reassociation value for the three strains was 100 % and was 25 % when the strains were compared with DNA from R. fulvus LMG 23003T. The strains had a mean DNA G+C content of 67.6 mol%. On the basis of their phylogenetic, genomic and phenotypic properties, the three strains represent a novel species within the genus Rhodanobacter, for which the name Rhodanobacter spathiphylli sp. nov. is proposed. The type strain is strain B39T (=LMG 23181T=DSM 17631T).

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3