Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China

Abstract

A Gram-positive, moderately halophilic, spore-forming bacterium, designated strain CG-15T, was isolated from a soda lake, Lake Chagannor, in the Inner Mongolia Autonomous Region, China. The cells were found to be motile short rods with ellipsoidal, terminal and deforming endospores. Strain CG-15T, a facultatively anaerobic bacterium, grew at pH 5.8–11.0 (optimally at pH 8.5), at 6–40 °C (optimally at 37 °C) and at salinities of 3–20 % (w/v) total salts (optimally at 7 % w/v). On the basis of the results of 16S rRNA gene sequence analysis, strain CG-15T was shown to belong to the genus Bacillus (phylum Firmicutes), showing the greatest phylogenetic similarity with respect to Bacillus saliphilus (96.0 %). The DNA G+C content of the novel isolate was found to be 53.8 mol%. The major cellular fatty acids of strain CG-15T were anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0, and its polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three different unidentified phospholipids. The analysis of the quinones showed that MK-7 was the major menaquinone. The peptidoglycan type was A1γ, with meso-diaminopimelic acid as the diagnostic diamino acid. On the basis of the data from this polyphasic study, strain CG-15T represents a novel species of the genus Bacillus, for which the name Bacillus chagannorensis sp. nov. is proposed. The type strain is CG-15T (=CCM 7371T=CECT 7153T=CGMCC 1.6292T=DSM 18086T).