Ureibacillus composti sp. nov. and Ureibacillus thermophilus sp. nov., isolated from livestock-manure composts

Abstract

Two Gram-negative, rod-shaped, thermophilic bacterial strains, HC145Tand HC148T, were isolated from a compost sample from a compost facility in Ichon, Korea. Sequencing of the 16S rRNA genes of HC145Tand HC148Tand comparative analyses of the resulting sequences clearly showed that these strains had a phylogenetic affiliation to the genusUreibacillus. The level of 16S rRNA similarity between the two novel strains was 98.4 % and the levels of sequence similarity between them and existingUreibacillusspecies were 97.8–98.1 (HC145T) and 97.4–98.7 % (HC148T). The DNA–DNA reassociation values between the two strains and the type strains ofUreibacillusspecies ranged from 38 to 51 %. The polar lipid profiles for both isolates consisted of phosphatidylglycerol, diphosphatidylglycerol, phospholipids and glycolipids of unknown composition. The major quinones were MK-8, MK-9 and MK-7, the peptidoglycan type wasl-Lys←d-Asp and the main cellular fatty acid was iso-C16 : 0. The DNA G+C contents of strains HC145Tand HC148Twere 42.4 and 38.5 mol%, respectively. On the basis of the data from this polyphasic study, strains HC145Tand HC148Trepresent members of the genusUreibacillus, for which the namesUreibacillus compostisp. nov. andUreibacillus thermophilussp. nov., respectively, are proposed. The type strain ofU. compostiis HC145T(=KACC 11361T=DSM 17951T) and the type strain ofU. thermophilusis HC148T(=KACC 11362T=DSM 17952T).