Characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, Serratia and Pantoea

Author:

Evans T. J.1,Crow M. A.1,Williamson N. R.1,Orme W.1,Thomson N. R.1,Komitopoulou E.2,Salmond G. P. C.1

Affiliation:

1. Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, UK

2. Leatherhead Food International Limited, Randalls Road, Leatherhead KT22 7RY, UK

Abstract

A phage (ΦOT8) isolated onSerratiasp. ATCC 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10−4transductants per p.f.u.). ΦOT8 was shown to have a broad host range because it also infected a strain ofPantoea agglomeransisolated from the rhizosphere. Transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of genes between bacterial genera.Serratiasp. ATCC 39006 andP. agglomeransproduce a number of interesting secondary metabolites that have potential applications in cancer therapy and biocontrol of fungal infections. ΦOT8 has utility as a powerful functional genomics tool in these bacteria.

Publisher

Microbiology Society

Subject

Microbiology

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