Monocyte-derived dendritic cells are permissive to the complete replicative cycle of human cytomegalovirus-Reference-Cited by-同舟云学术

Monocyte-derived dendritic cells are permissive to the complete replicative cycle of human cytomegalovirus

Published:2000-02-01 Issue:2 Volume:81 Page:393-399
ISSN:1350-0872
Container-title:Microbiology
language:en
Short-container-title:

Author:

Riegler S.¹,Hebart H.²,Einsele H.²,Brossart P.²,Jahn G.¹,Sinzger C.¹

Affiliation:

1. Department of Medical Virology, University of Tübingen, Calwerstrasse 7/6, D-72076 Tübingen, Germany1

2. Department of Medicine, University of Tübingen, Tübingen, Germany2

Abstract

The susceptibility of monocyte-derived immature dendritic cells (DC) to infection by various strains of human cytomegalovirus (HCMV) was analysed. Immature DC were generated by incubation of peripheral blood monocytes with interleukin-4 and granulocyte–macrophage colony-stimulating factor for 7 days and were characterized by a CD1a+/CD40+/CD80+/CD86+/HLA-DR+/CD14− phenotype. Viral antigen expression and production of infectious progeny virus were analysed in infected immature DC cultures. Immature DC were 80–90 % susceptible to HCMV strains that had been propagated in endothelial cell culture, whereas the infection rate was negligible with fibroblast-adapted HCMV strains. Immature DC infection resulted in expression of viral immediate early, early and late genes. Productive infection was proven by the detection of infectious virus in single-step growth curves and in infectious centre assays. It is concluded that HCMV might interfere with the host immune reaction by permissive, lytic infection of immature DC.

Publisher

Microbiology Society

Subject

Microbiology

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