Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods-Reference-Cited by-同舟云学术

Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods

Published:2006-01-01 Issue:1 Volume:55 Page:127-131
ISSN:0022-2615
Container-title:Journal of Medical Microbiology
language:en
Short-container-title:

Author:

Suffys Philip,da Silva Rocha Adalgiza,Brandão Adeilton,Vanderborght Bart,Mijs Wouter¹,Jannes Geert¹,Mello Fernanda C. Q.,Pedro Heloisa da Silveira Paro²,de Souza Fonseca Leila,de Oliveira Rosângela Siqueira³,Cardoso Leão Sylvia³,Féres Saad Maria Helena

Affiliation:

1. Innogenetics N. V., Technologiepark 6, B-9052 Ghent, Belgium

2. Adolfo Lutz Institute, São Paulo, Brazil

3. Department of Microbiology, Immunology and Parasitology, Paulista School of Medicine, São Paulo, Brazil

Abstract

Three mycobacterial isolates, one from the blood of an HIV-infected patient and two consecutive isolates from a woman with unknown HIV status, had been identified as belonging to the Mycobacterium avium complex by conventional procedures. In both patients, using genetic analysis procedures such as PCR–restriction enzyme analysis (PRA) of the hsp65 gene, a commercially available reverse hybridization-based assay (INNO-LiPA mycobacteria) and/or sequencing analysis of the 16S–23S internal transcribed spacer (ITS), the presence of Mycobacterium lentiflavum was also demonstrated. At the time of detection, both cases were also infected with M. avium, suggesting an underestimation of infection with M. lentiflavum and co-infection with different Mycobacterium species.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

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