Affiliation:
1. GSK Biologicals, Rixensart, Belgium
2. Medicago Inc., Québec, Québec, Canada
3. GSK Biologicals, Laval, Québec, Canada
4. University of Glasgow, Glasgow, UK
Abstract
Restricted to the genusStreptococcus, the Pht protein family comprises four members: PhtA, PhtB, PhtD and PhtE. This family has the potential to provide a protein candidate for incorporation in pneumococcal vaccines. Based on sequence analysis and on RT-PCR experiments, we show here that thephtgenes are organized in tandem but that their expression, except that ofphtD, is monocistronic. PhtD, PhtE, PhtB and PhtA are present in 100, 97, 81 and 62 % of the strains, respectively, and, by analysing its sequence conservation across 107 pneumococcal strains, we showed that PhtD displays very little variability. To analyse the physiological function of these proteins, several mutants were constructed. The quadruple Pht-deficient mutant was not able to grow in a poor culture medium, but the addition of Zn2+or Mn2+restored its growth capacity. Moreover, thephtDmRNA expression level increased when the culture medium was depleted in zinc. Therefore, we suggest that these proteins are zinc and manganese scavengers, and are able to store these metals and to release them when the bacterium faces an ion-restricted environment. The data also showed that this protein family, and more particularly PhtD, is a promising candidate to be incorporated into pneumococcal vaccines.
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62 articles.
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