The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice

Author:

Anisimov Andrey P.1,Bakhteeva Irina V.1,Panfertsev Evgeniy A.1,Svetoch Tat'yana E.1,Kravchenko Tat'yana B.1,Platonov Mikhail E.1,Titareva Galina M.1,Kombarova Tat'yana I.1,Ivanov Sergey A.1,Rakin Alexander V.2,Amoako Kingsley K.3,Dentovskaya Svetlana V.1

Affiliation:

1. State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Serpukhov District, Moscow Region, Russia

2. Max von Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie, Pettenkofer Str. 9a, 80336 Munich, Germany

3. Canadian Food Inspection Agency Lethbridge Laboratory, PO 640, Township Road 9-1, Lethbridge, AB T1J 3Z4, Canada

Abstract

Two isogenic sets of Yersinia pestis strains were generated, composed of wild-type strains 231 and I-1996, their non-polar pH 6 mutants with deletions in the psaA gene that codes for its structural subunit or the whole operon, as well as strains with restored ability for temperature- and pH-dependent synthesis of adhesion pili or constitutive production of pH 6 antigen. The mutants were generated by site-directed mutagenesis of the psa operon and subsequent complementation in trans. It was shown that the loss of synthesis or constitutive production of pH 6 antigen did not influence Y. pestis virulence or the average survival time of subcutaneously inoculated BALB/c naïve mice or animals immunized with this antigen.

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

Reference76 articles.

1. Molecular genetic mechanisms of the formation and functional significance of the capsule of Yersinia pestis;Anisimov,1999

2. Factors of Yersinia pestis providing circulation and persistence of plague pathogen in ecosystems of natural foci. Communication 2;Anisimov;Mol Gen Mikrobiol Virusol,2002a

3. Yersinia pestis factors, assuring circulation and maintenance of the plague pathogen in natural foci ecosystems;Anisimov;Mol Gen Mikrobiol Virusol,2002b

4. Intraspecific diversity of Yersinia pestis;Anisimov;Clin Microbiol Rev,2004

5. Variability of the protein sequences of LcrV between epidemic and atypical rhamnose-positive strains of Yersinia pestis;Anisimov;Adv Exp Med Biol,2007

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3