Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens-Reference-Cited by-同舟云学术

Molecular detection of Mycobacterium tuberculosis in poor-quality cough specimens

Published:2020-09-01 Issue:9 Volume:69 Page:1179-1182
ISSN:0022-2615
Container-title:Journal of Medical Microbiology
language:en
Short-container-title:

Author:

Mboneni Tondani A.¹^ORCID,Eales Owen O.²^ORCID,Mosina Ntsoaki L.³^ORCID,Fourie P. Bernard¹^ORCID

Affiliation:

1. Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa

2. Department of Family Medicine, University of Pretoria, Pretoria, South Africa

3. Department of Genetics, University of Pretoria, Pretoria, South Africa

Abstract

Clinical specimens unfit for laboratory processing represent missed opportunities for diagnosing tuberculosis. Poor-quality cough specimens (n=61) from presumptive tuberculosis cases were cultured and GeneXpert MTB/RIF (Xpert) successfully performed on samples transferred by flocked swab into PrimeStore molecular transport medium (PS-MTM).

Mycobacterium tuberculosis

was grown in culture from 13 (21.3 %) and Xpert reported 15 (24.2 %) positive, of which 10 concordant. RT-PCR of PS-MTM samples showed enhanced sensitivity; three positives were missed by Xpert, five by culture and three more detected for a total of 21 positives (34.4 %).

Funder

National Research Foundation

Publisher

Microbiology Society

Subject

Microbiology (medical),General Medicine,Microbiology

Reference9 articles.

1. NICD laboratory Handbook. standard operating procedure. document NIC0104 version 12

2. Laboratory evaluation of a specimen transport medium for downstream molecular processing of sputum samples to detect Mycobacterium tuberculosis

3. Molecular detection of Mycobacterium tuberculosis from sputum transported in PrimeStore^® from rural settings

4. Field evaluation of a novel preservation medium to transport sputum specimens for molecular detection ofMycobacterium tuberculosisin a rural African setting

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