Frateuria terrea sp. nov., isolated from forest soil, and emended description of the genus Frateuria

Abstract

A bacterial strain, designated VA24T, was isolated from forest soil of the Changbai Mountains, Heilongjiang province, China. Cells of strain VA24Twere Gram-reaction-negative, aerobic, non-spore-forming, long rods, 0.3–0.5×2.0–3.0 μm in size and were motile by means of a subpolar flagellum. Strain VA24Twas oxidase-positive and catalase-negative. Growth occurred at 21–36 °C, pH 5–10 and in 0–2 % (w/v) NaCl but did not occur at 37 °C. The predominant respiratory quinone was Q-8, the major polar lipids were phosphatidylethanolamine and phosphatidylmonomethylethanolamine and the major cellular fatty acids were iso-C15 : 0(14.9 %), iso-C17 : 1ω9c(14.1 %), iso-C17 : 0(10.8 %) and iso-C16 : 0(10.3 %). The DNA G+C content was 67.4 mol% (Tm). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain VA24Twas closely related toFrateuria aurantiaIFO 13300Twith 96.9 % sequence similarity. DNA–DNA relatedness of strain VA24TtoF. aurantiaDSM 6220Twas 15.8 %. Based on its phenotypic and genotypic features, strain VA24Trepresents a novel species of the genusFrateuria, for which the nameFrateuria terreasp. nov. is proposed. The type strain is VA24T(=CGMCC 1.7053T=NBRC 104236T).