Affiliation:
1. School of Pharmacy, Quanzhou Medical College, Quanzhou, PR China
2. College of Oceanology and Food Science, Quanzhou Normal University, Quanzhou, PR China
3. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, PR China
4. Fujian Province Key Laboratory for the Development of Bioactive Material from Marine Algae, Quanzhou, PR China
Abstract
A Gram-stain-negative, rod-shaped and light yellow-pigmented strain designated MBT5T was isolated from tidal flat sediment of an oyster farming area in Quanzhou Bay, PR China. Catalase activity and oxidase activity were positive. Flexirubin-type pigment was absent. Growth was observed at 10–40 °C (optimum, 35 °C), pH 6–9 (optimum, pH 7), and with 1–7 % NaCl (optimum, 2 %, w/v). The 16S rRNA gene of strain MBT5T had maximum sequence similarity values with
Meridianimaribacter flavus
NH57NT,
Mangrovimonas yunxiaonensis
LYYY01T and Mangrovimonas futianensis AS18T of 95.6, 95.4 and 94.9 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences and 120 conserved concatenated proteins indicated that strain MBT5T was affiliated to the genus
Mangrovimonas
and formed a distinct monophyletic branch. The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values between strain MBT5T and the type strains of
Mangrovimonas
were estimated to be 17.3–18.7 %, 70.9–71.5 % and 66.4–68.2 %, respectively. The respiratory quinone was menaquinone-6. The major fatty acids were iso-C15 : 0 and iso-C15 : 1 G. The draft genome size was 2 952 053 bp with a DNA G+C content of 36.5 %. Based on phenotypic, physiological, phylogenetic and genomic data, together with chemotaxonomic characteristics, strain MBT5T represents a novel species, for which the name Mangrovimonas aestuarii sp. nov. is proposed. The type strain is MBT5T (=MCCC 1K06186T=KCTC 92888T=GDMCC 1.3851T).
Funder
Key Science and Technology Projects of Quanzhou Medical College
Quanzhou City Science & Technology Program of China
National Science and Technology Fundamental Resources Investigation Program of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology