Mangrovimonas futianensis sp. nov., a novel species isolated from mangrove sediment

Abstract

Three bacterial strains, designated as AS18T, AS27 and AS39, were obtained from mangrove sediment sampled in Futian district, Shenzhen, PR China. Cells of these strains were Gram-negative rods with no flagella. They were able to grow at 10–42 °C (optimum, 37 °C), at pH 5–9 (optimum, pH 6) and in 1–11 % (w/v) NaCl (optimum, 2 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolates were clustered within the genus Mangrovimonas , closely related to Mangrovimonas yunxiaonensis (95.1 % similarity) and Mangrovimonas spongiae (94.7 % similarity). Phylogenomic analysis based on multiple core genes revealed that the three strains were located in a different cluster from other closely related strains of the genus Mangrovimonas . Digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values calculated from genome sequences between isolates and type strains were lower than 25, 75 and 72 %, respectively. The dominant fatty acids were iso-C15 : 0 and iso-C15 : 1 G. The main respiratory quinone was identified as MK-6. The major polar lipids contained phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. The results of multiphase taxonomy suggested that the three strains should be assigned to a novel species of the genus Mangrovimonas , for which the name Mangrovimonas futianensis sp. nov. is proposed, with the type strain AS18T (=GDMCC 1.2739T=JCM 34871T).