Affiliation:
1. Wuhan Pulmonary Hospital, Wuhan Institution of Tuberculosis Control, Wuhan 430030, PR China
2. National Clinical Laboratory on Tuberculosis, Beijing Key Laboratory on Drug-Resistant Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing 101149, PR China
Abstract
A slow-growing, scotochromogenic mycobacterial strain (24T) was isolated from the sputum of a Chinese male human. Phylogenetic analysis using the 16S rRNA gene assigned strain 24T to the
Mycobacterium gordonae
complex, which includes
Mycobacterium gordonae
and
Mycobacterium paragordonae
. The phenotypic characteristics, unique mycolic acid profile and the results of phylogenetic analysis based on hsp65 and rpoB sequences strongly supported the taxonomic status of strain 24T as a representative of a species distinct from the other members of the
M. gordonae
complex. The genomic G+C content of strain 24T was 65.40mol%. Genomic comparisons showed that strain 24T and
M. gordonae
ATCC 14470T had an average nucleotide identity (ANI) value of 81.00 % and a DNA–DNA hybridization (DDH) value of 22.80 %, while the ANI and DDH values between strain 24Tand
M. paragordonae
49 061T were 80.98 and 22.80 %, respectively. In terms of phylogenetic, phenotypic and chemotaxonomic features, strain 24T is distinguishable from its closest phylogenetic relatives and represents a novel species of the genus
Mycobacterium
, therefore the name Mycobacterium vicinigordonae sp. nov. is proposed. The type strain is 24T (=CMCC 93559T=DSM 105979T).
Funder
Infectious Diseases Special Project, Ministry of Health of China
Innovative Research Group Project of the National Natural Science Foundation of China
Municipal Science & Technology commission
Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support
Natural Science Foundation of Hubei Province
Chinese Society of Clinical Oncology
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
7 articles.
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