Affiliation:
1. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China
2. Nanjing Customs, Nanjing, Jiangsu 210001, PR China
3. College of Basic Medical Science, Jiujiang University, Jiujiang, Jiangxi, PR China
Abstract
A Gram-stain-negative, rod-shaped, non-motile and non-spore-forming bacterium, designated HAL-9T, was isolated from oil-contaminated soil in Daqing oilfield, Heilongjiang Province, PR China. Strain HAL-9T was able to degrade quizalofop-p-ethyl and diclofop-methyl. Growth was observed at 10–35 °C (optimum, 30 °C), pH 6.0–10.0 (optimum, pH 7.0) and salinity of 0 %–5.0 % (w/v; optimum 1.0 %). The results of phylogenetic analysis based on the 16S rRNA gene indicated that strain HAL-9T belongs to the genus
Sphingobacterium
and showed the highest sequence similarity (98.3 %) to
Sphingobacterium alkalisoli
Y3L14T, followed by
Sphingobacterium mizutaii
DSM 11724T (95.1 %) and
Sphingobacterium lactis
DSM 22361T (95.1 %). Menaquinone-7 (MK-7) was the only isoprenoid quinone. The predominant cellular fatty acids were summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c), iso-C15: 0 and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, three phosphoglycolipids and three unidentified lipids. The draft genome of strain HAL-9T was 5.41 Mb. The G+C content of strain HAL-9T was 40.6 mol%. Furthermore, the average nucleotide identity and in silico DNA–DNA hybridization values between strain HAL-9T and
S. alkalisoli
Y3L14T were 86.2 % and 32.8 %, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, genotypic and phylogenetic evidence, strain HAL-9T represents a novel species in the genus
Sphingobacterium
, for which the name Sphingobacterium olei sp. nov. is proposed. The type strain is HAL-9T (=ACCC 61581T=CCTCC AB 2019176T=KCTC 72287T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
7 articles.
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