Salinigranum halophilum sp. nov., isolated from marine solar salterns

Abstract

Three halophilic archaeal strains, YJ-53T, ZS-5 and DYF38, were isolated from marine solar salterns located in different provinces of China. The three strains formed a single cluster (99.7–99.8 and 97.9–99.2 % similarities, respectively) that was separate from the current two members of Salinigranum (96.7–98.0 and 89.8–92.9 % similarities, respectively) on the basis of 16S rRNA and rpoB′ gene sequence comparisons and phylogenetic analysis. Diverse phenotypic characteristics differentiated strains YJ-53T, ZS-5 and DYF38 from Salinigranum rubrum GX10T and Salinigranum salinum YJ-50-S2T. The major polar lipids of isolated strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and two major glycolipids chromatographically identical to mannosyl glucosyl diether and sulfated mannosyl glucosyl diether, detected in the current members of Salinigranum . The OrthoANI and in silico DNA–DNA hybridization (DDH) values between the three strains were in the range of 97.7–98.4 % and 80.3–86.1 %, respectively, much higher than the threshold values proposed as species boundaries (average nucleotide identity 95–96 % and in silico DDH 70 %), revealing that the three strains represent one species. Results of comparative OrthoANI and in silico DDH analyses of the strains described in this study with validly described members of the genus Salinigranum supported that strains YJ-53T (=CGMCC 1.12860T=JCM 30238T), ZS-5 (=CGMCC 1.12867=JCM 30240) and DYF38 (=CGMCC 1.13779=JCM 33557) represent a novel species of the genus Salinigranum , for which the name Salinigranum halophilum sp. nov. is proposed.