Lysobacter psychrotolerans sp. nov., isolated from soil in the Tianshan Mountains, Xinjiang, China

Abstract

A novel aerobic bacterial strain, designated ZS60T, with long, rod-shaped, Gram-staining-negative, aerobic cells was isolated from the soil in the Tianshan Mountains, Xinjiang, China. Phylogenetic analysis based on its 16S rRNA gene sequence indicated that strain ZS60T was affiliated with the genus Lysobacter , and was most closely related to Lysobacter daejeonensis GH1-9T (96.9 %), Lysobacter caeni BUT-8T (96.8 %) and Lysobacter ruishenii CTN-1T (96.7 %). The average nucleotide identity values between strain ZS60T, L. daejeonensis GH1-9T and L. ruishenii CTN-1T were 78.14 and 78.39 %, respectively. The DNA–DNA relatedness between strain ZS60T, L. daejeonensis GH1-9T and L. caeni BUT-8T were 44.8 and 39.1 %, respectively. The genomic DNA G+C content of the strain ZS60T was 67.7 mol% (draft genome sequence), and Q-8 was the predominant ubiquinone. The major cellular fatty acids of strain ZS60T were iso-C15 : 0 (23.4 %), iso-C17 : 0 (17.2 %) and iso-C17 : 1  ω9c (12.6 %). On the basis of genotypic, phenotypic and biochemical data, strain ZS60T is considered to represent a novel species of the genus Lysobacter , for which the name Lysobacter psychrotolerans sp. nov. is proposed. The type strain is ZS60T (=CGMCC 1.15509T=NBRC 112614T).