Affiliation:
1. Department of Systems Biotechnology, Chung-Ang University, Anseong 456-756, Republic of Korea
Abstract
A novel bacterial strain, designated CJ29T, was isolated from ginseng soil of Anseong in South Korea. Cells of strain CJ29T were Gram-stain-negative, facultatively anaerobic, rod-shaped and non-motile. Strain CJ29T grew optimally at 28–30 °C and pH 7.0. Based on 16S rRNA gene sequence analysis, strain CJ29T was shown to belong to the genus
Lysobacter
within the class
Gammaproteobacteria
and was related most closely to
Lysobacter soli
DCY21T (98.5 % similarity) and
Lysobacter niastensis
GH41-7T (98.2 %). DNA–DNA relatedness between strain CJ29T and its closest relatives was below 55.6 %. The predominant cellular fatty acids of strain CJ29T were iso-C15 : 0, iso-C16 : 0 and iso-C17 : 1ω9c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major isoprenoid quinone was ubiquinone 8 (Q-8). The G+C content of the genomic DNA was 65.6 mol%. Phenotypic, genotypic and phylogenetic characteristics strongly supported the differentiation of strain CJ29T from related species of the genus
Lysobacter
. On the basis of data from this polyphasic taxonomic study, strain CJ29T is considered to represent a novel species of the genus
Lysobacter
, for which the name
Lysobacter
panacisoli sp. nov. is proposed. The type strain is CJ29T ( = KACC 17502T = JCM 19212T).
Funder
National Institute of Biological Resources
Ministry of Education, Science and Technology
National Research Foundation of Korea
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Reference29 articles.
1. Purification, bacteriolytic activity, and specificity of β-lytic protease from Lysobacter sp. IB-9374;Ahmed;J Biosci Bioeng,2003
2. Review and re-analysis of domain-specific 16S primers;Baker;J Microbiol Methods,2003
3. Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio;Christensen;Int J Syst Bacteriol,1978
4. Isoprenoid quinone analysis in classification and identification;Collins,1985
5. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains;Ezaki;Int J Syst Evol Microbiol,1989
Cited by
35 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献