Affiliation:
1. Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University, Seoul 02841, Republic of Korea
2. School of Earth and Environmental Science & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea
3. Department of Biotechnology, School of Life Science and Biotechnology, Korea University, Seoul 02841, Republic of Korea
Abstract
A novel Gram-stain-negative, rod-shaped, cream-coloured, motile, halotolerant bacterium, designated as YJPS3-2T, was isolated from saltern sediment of the Yellow sea in Yongyu-do, Republic of Korea. Strain YJPS3-2T grew at pH 5.0–10.0 (optimum, pH 7.0), 4–40 °C (optimum, 30 °C) and with 1–15% (w/v) NaCl (optimum 3 %). The 16S rRNA gene sequence analysis indicated that strain YJPS3-2T was closely related to those of
Halomonas halophila
F5-7T (98.75 %),
Halomonas salina
F8-11T (98.74 %),
Halomonas smyrnensis
AAD6T (98.66 %),
Halomonas organivorans
G-16.1T (98.34 %),
Halomonas koreensis
SS20T (97.98 %) and
Halomonas beimenensis
NTU-107T (96.93 %). The average nucleotide identity and digital DNA–DNA hybridization values between YJPS3-2T and related type strains were 86.9–91.6 % and 32.0–44.8 %. Strain YJPS3-2T was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C16 : 0 (31.4 %), C19 : 0
ω8c cyclo (16.3 %), C17 : 0 cyclo (11.9 %) and C12 : 0 3-OH (10.4 %). The polar lipids consisted of phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain YJPS3-2T is 68.1mol %. Based on the polyphasic taxonomic evidence presented in this study, YJPS3-2T should be classified as representing a novel species within the genus Halmonas, for which name Halomonas getboli is proposed, with the type strain YJPS3-2T (= KCTC 92124T=KACC 22561T=JCM 35085T).
Funder
Development of living shoreline technology based on blue carbon science toward climate change adaptation
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
3 articles.
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