Affiliation:
1. Department of Environmental Engineering, College of Science and Technology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 30019, Republic of Korea
2. Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi-Do 16227, Republic of Korea
3. Department of Biological Science, College of Science and Engineering, Sangji University, Wonju, Republic of Korea
Abstract
Two yellow-pigmented, non-motile, Gram-stain-negative, and rod-shaped bacteria, designated TW-4T and TNP-2 were obtained from oil-contaminated soil. Both strains degrade diesel oil, hydrolyse aesculin, DNA, Tween 40 and Tween 60. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain TW-4T formed a lineage within the family
Erythrobacteraceae
and clustered as members of the genus
Novosphingobium
. The closest members of strain TW-4T were
Novosphingobium subterraneum
DSM 12447T (97.9 %, sequence similarity),
Novosphingobium lubricantis
KSS165-70T (97.8 %),
Novosphingobium taihuense
T3-B9T (97.8 %),
Novosphingobium aromaticivorans
DSM 12444T (97.7 %),
Novosphingobium flavum
UCT-28T (97.7 %), and
Novosphingobium bradum
STM-24T (97.6 %). The sequence similarity for other members was ≤97.6 %. The genome of strain TW-4T was 4 683 467 bp long with 44 scaffolds and 4280 protein-coding genes. The sole respiratory quinone was Q-10. The major cellular fatty acids were summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c), summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c), C16 : 0 and C14 : 0 2-OH. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylcholine (PC), phosphatidyl-n-methylethanolamine (PME) and sphingoglycolipid (SGL). The DNA G+C content of the type strain was 65.0 %. The average nucleotide identity (ANIu) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain TW-4T and closest members were below the threshold value for species delineation. Based on polyphasic taxonomic analyses, strain TW-4T represents novel species in the genus
Novosphingobium
, for which the name Novosphingobium olei sp. nov. is proposed. The type strain is TW-4T (=KACC 21628T=NBRC 114364T) and strain TNP-2 (=KACC 21629=NBRC 114365) represents an additional strain. Based on new data obtained in this study, it is also proposed to reclassify
Novosphingobium stygium
as a later heterotypic synonym of
Novosphingobium aromaticivorans
.
Funder
National Research Foundation
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
4 articles.
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