Affiliation:
1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China
Abstract
A novel Gram-stain-negative, aerobic and rod-shaped halophilic archaeon, designated HD8-45T, was isolated from the red brine of salted brown alga Laminaria produced at Dalian, PR China. According to the results of 16S rRNA gene and rpoB′ gene sequence comparisons, strain HD8-45T showed the highest sequence similarity to the corresponding genes of
Salinirussus salinus
YGH44T (95.1 and 85.2 % similarities, respectively),
Halovenus aranensis
EB27T (91.2 and 86.0 % similarities, respectively). The low sequence similarity and the phylogeny implied the novel generic status of strain HD8-45T. Genomic relatedness analyses showed that strain HD8-45T were clearly distinguished from other species in the order
Halobacteriales
, with average nucleotide identity, amino acid identity and in silico DNA–DNA hybridization values not more than 75.1, 65.6 and 21.5 %. The polar lipid pattern contained phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, two major glycolipids and two minor glycolipids. The two major glycolipids and a minor glycolipid were chromatographically identical to disulfated mannosyl glucosyl diether, sulfated mannosyl glucosyl diether and mannosyl glucosyl diether, respectively. The major respiratory quinones were menaquinone MK-8 and MK-8(H2). The DNA G+C content was 62.0 mol% (Tm
) and 61.9 mol% (genome). All these results showed that strain HD8-45T represents a novel species of a new genus in the order
Halobacteriales
, for which the name Salinibaculum litoreum gen. nov., sp. nov. is proposed. The type strain of Salinibaculum litoreum is HD8-45T (=CGMCC 1.15328T=JCM 31107T).
Funder
National Natural Science Foundation of China
National Science & Technology Infrastructure Program of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
6 articles.
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