Cellulases from Mycelial fungi Penicillium verruculosum as a Real Alternative to Trichoderma Enzymes in Industrial Hydrolysis of Cellulosic Biomass

Abstract

Abstract The possibility of using the recipient strain Penicillium verruculosum B1-537 (ΔniaD) as a producer of laboratory and industrial enzymes was considered. The advantage of this strain is its ability to secrete a basic cellulase complex consisting of cellobiohydrolases, endoglucanases, and β-glucosidase, which exceeds in its hydrolytic ability the enzyme complex of Hypocrea (Trichoderma) strains. Using the expression system, the basic complex of cellulases of the recipient strain Piptochaetium verruculosum B1-537 (ΔniaD) was supplemented with new (booster) enzymes that are necessary to increase its hydrolytic activity. Enzyme preparations adapted to the processing of various types of renewable plant biomass were obtained.