Improved photosystem II and defense enzymes activity in rice (Oryza sativa) by biopriming against Xanthomonas oryzae pv. oryzae

Abstract

Bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo) is a major threat to rice production as it accounts for loss up to 50% of annual rice grain yield. Xoo causes leaf tissue necrosis and as a result there is downregulation of the photosynthetic mechanisms of plant. Measurement of chl a fluorescence is an easy, fast, non-invasive and highly sensitive technique that can be used to understand plant health by measuring alterations in PSII activity, in response to different biotic and abiotic stresses. In the present investigation, rice seeds were bio-primed with two bacterial spp. (e.g. Bacillus subtilis and B. megaterium) and one fungal strain (i.e. Trichoderma erinaceum). The induction of defence against BB disease by bio-priming with biotic agents, was studied as response in alteration of PSII and defence enzymes activity. Field experiment was conducted with the best biotic agent; i.e. B. subtilis obtained from all experiments to study whether defence induction by it have any side effect on rice yield and biomass. Net house and field experiments have suggested that among all the biotic agents studied, bio-priming of rice seeds with B. subtilis has protected the photosynthetic machinery of plants from being damaged by BB without having any detrimental effect on rice grain yield.