Native Trichoderma Induced the Defense-Related Enzymes and Genes in Rice against Xanthomonas oryzae pv. oryzae (Xoo)
Author:
Islam Md. Rashidul1ORCID, Chowdhury Rabin1, Roy Arpita Saha1, Islam Md. Nazmul1, Mita Mamuna Mahjabin1, Bashar Samrin1, Saha Plabon1, Rahat Ridwan Ahmed2ORCID, Hasan Mehedi2, Akter Mst. Arjina1ORCID, Alam Md. Zahangir1ORCID, Latif Md. Abdul3
Affiliation:
1. Plant Bacteriology and Biotechnology Laboratory, Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh 2. Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh 3. Plant Pathology Division, Bangladesh Rice Research Institute, Joydebpur, Gazipur 1701, Bangladesh
Abstract
The application of Trichoderma is a form of biological control that has been effective in combating Xanthomonas oryzae pv. oryzae, the causative agent of the devastating disease known as bacterial blight of rice. In this present study, four strains of Trichoderma, viz., T. paraviridescens (BDISOF67), T. erinaceum (BDISOF91), T. asperellum (BDISOF08), and T. asperellum (BDISOF09), were collected from the rice rhizosphere and used to test their potentiality in reducing bacterial blight. The expression patterns of several core defense-related enzymes and genes related to SA and JA pathways were studied to explore the mechanism of induced resistance by those Trichoderma strains. The results primarily indicated that all Trichoderma were significantly efficient in reducing the lesion length of the leaf over rice check variety (IR24) through enhancing the expression of core defense-related enzymes, such as PAL, PPO, CAT, and POD activities by 4.27, 1.77, 3.53, and 1.57-fold, respectively, over control. Moreover, the results of qRT-PCR exhibited an upregulation of genes OsPR1, OsPR10, OsWRKY45, OsWRKY62, OsWRKY71, OsHI-LOX, and OsACS2 after 24 h of inoculation with all tested Trichoderma strains. However, in the case of RT-PCR, no major changes in OsPR1 and OsPR10 expression were observed in plants treated with different Trichoderma strains during different courses of time. Collectively, Trichoderma induced resistance in rice against X. oryzae pv. oryzae by triggering these core defense-related enzymes and genes associated with SA and JA pathways.
Funder
Program Based Research Grant (PBRG), National Agricultural Technology-2 (NATP-2), BARC, Farmgate, Dhaka granted to Md. Rashidul Islam World Bank and IFAD Funded Project
Subject
Plant Science,Ecology,Ecology, Evolution, Behavior and Systematics
Reference89 articles.
1. Diversity in the Oryza genus;Vaughan;Curr. Opin. Plant Biol.,2003 2. Oryza Wild Species: An alternative for rice breeding under abiotic stress conditions;Szareski;Am. J. Plant Sci.,2018 3. BBS (Bangladesh Bureau of Statistics) (2020). 2020: Yearbook of Agricultural Statistics, Bureau of Statistics and Informatics Division (SID), Ministry of Planning, Government of the People ‘s Republic of Bangladesh. 4. Estimation of rice yield loss using a simple linear regression model for bacterial blight disease;Ansari;Bangladesh Rice J.,2019 5. Lal, R., Sivakumar, M.V.K., Faiz, M.A., Rahman, A.H.M.M., and Islam, K.R. (2010). Climate Change and Food Security in South Asia, Springer.
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