Practical Isolation of Asperuloside from Coprosma quadrifida via Rapid Pressurised Hot Water Extraction

Abstract

The valuable glycoside asperuloside was efficiently isolated in multigram quantities from Coprosma quadrifida employing a recently developed rapid pressurised hot water extraction (PHWE) method that utilises an unmodified household espresso machine. This study represents the first time C. quadrifida has been extracted and its major chemical components isolated and analysed. Our findings reveal that this species is a rich source of asperuloside (7.0 % yield w/w). Significantly, this represents one of the highest yields obtained of this glycoside from a Coprosma species, and the entire Rubiaceae family more generally.