The Role of Photosynthesis in Flowering of the Long-Day Plant Sinapis alba

Abstract

Flowering can be induced in the long-day plant Sinapis alba in 8-h photoperiods provided that the irradiance is close to that at which leaf photosynthesis is light-saturated (e.g. 96 J m-2 s-1). Three such 8-h cycles result in 10% flowering and six are required for full flowering, whereas only one long-day cycle of 16-20 h duration at a much lower irradiance (25 J m-2 s-1) is required for full flowering. High irradiance during the single long day promotes flowering when given for the first 8 h of a 16-h photoperiod, but is inhibitory over the last 8 h. Photosynthetic CO2 uptake is crucial for this response to high irradiance, as both its inhibitory and promotive effects on flowering are reversed by the removal of atmospheric CO2 during the period of high irradiance. Compared with plants kept in short days (8-h photoperiod), export of 14C-labelled assimilates from the leaf during a 24-h period was only 50-60% greater in plants exposed to a long day (20-h photoperiod), because plants in short days compensated to a degree for their shorter photosynthetic period by mobilizing leaf reserves during darkness. However, flowering can occur with no evident enhancement of supply of assimilate to the shoot apex, for example following dis- placement of the short day or on removal of atmospheric CO2 during the last 12 h of exposure to a 20-h long day. Also, the flowering response to radiant flux density during the second half of a long day shows an optimum between 15 and 70 J m-2 s-1, with reduced flowering both above and below this irradiance. Thus, although there is no absolute requirement for long days to induce flowering in S. alba, light reactions cther than photosynthesis probably contribute to photoperiodic induction in this species.