Abstract
Cattle morulae and blastocysts were frozen, stored in liquid N2 and then warmed and cultured in vitro at 37� 5�C. They were frozen and stored in Dulbecco phosphate buffer enriched with 25 % cattle serum (DB+S)and containing 1�5 M dimethylsulphoxide (DMSO) or 1�0 M glycerol. Embryos were cooled at rates of 0 �13, 0 �15 or O� 30�Cjmin and warmed at rates of 1 �2, 2�2 or 4� 6�C/min (measured over the range 0 to - 50�C). Warming and cooling between 0 and 37� 5�C was at a rate of 0�7�C/min and, after warming, embryos were cultured in DB+S for 12-18 h. A number of embryos were transferred after culture to recipient cows.
Subject
Developmental Biology,Endocrinology,Genetics,General Materials Science,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,General Medicine,Biotechnology
Cited by
23 articles.
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