Ecotypic variation between and within two populations of Trifolium tomentosum (woolly clover) from Syria and Western Australia: its success as a colonising species

Abstract

Ecotypic variation was studied between and within populations of Trifolium tomentosum (woolly clover) using seed that was collected from 2 semi-arid environments: Tel Hadya, Syria, and Pingrup, Western Australia. The seed was collected from 64 subplots within a 40-m2 grid at each site and the material was grown at the University of Western Australia Field Station at Shenton Park, Perth. Fifteen morphological characters were scored and were analysed using analysis of variance, principal components analysis, and discriminant function analysis. Material from the 2 sites was separated using multivariate analysis, with the seed from Tel Hadya containing more within-site variation. It is suggested that the lack of within-site variation observed at Pingrup is the result of a number of factors: a limited amount of genetic diversity being present in the original introduced material, a preference of T. tomentosum for alkaline soils rather than the acid soils predominantly occurring in Western Australia, the harsh selection pressures present in a semi-arid environment, and a limited time for genotypes to adapt to specific micro-niches within each environment. The results are used to contribute to our understanding of the success of colonising species in semi-arid environments.