Deteksi dan Respons Lima Varietas Pepaya terhadap Tiga Isolat Papaya Ringspot Virus (PRSV)

Abstract

<p>Papaya (Carica papaya L.) is one of favorite fruits for Indonesian people and known as a good vitamin source. Recently, a ring<br />spot disease was reported in some growing areas and it potentially caused yield loss. The disease is caused by Papaya<br />ringspot virus (PRSV) and considered as a new disease in Indonesia. Therefore, preliminary research was done to study the<br />response of some varieties of papaya against PRSV infection. Field isolates of PRSV were collected from Medan, Aceh, and<br />Bogor. Identification of the isolates was done based on the nucleotide sequence analysis of coat protein gene. Response of<br />papaya varieties was evaluated based on the results of mechanical inoculation. Identification results showed that the<br />nucleotide sequences of PRSV isolates Medan, Aceh, and Bogor, have a high similarity to that from other countries, i.e.<br />Thailand, Australia, China, Japan, Vietnam, and Taiwan (93.0–98.3%). Disease incidence reached 100% for all isolates of PRSV<br />on all test varieties, although based on the DIBA’s result, it was known that the virus titer in plants are different. No varieties of<br />papaya showed resistant to PRSV.</p>