Affiliation:
1. Lampung University
2. National Research and Innovation Agency
Abstract
Abstract
Begomovirus attacks on papaya plants have been identified in several regions in Indonesia. Detection based on viral nucleic acid in each category of papaya plants in Tanggamus, Pringsewu, West Lampung, Pesawaran, Central Lampung, and Bandar Lampung using universal primers SPG1/SPG2 showed positive results for Begomovirus infection with visible DNA bands measuring ± 900 bp. Direct nucleotide sequencing using SPG1/SPG2 primers for the AC2 dan AC1 genes of the Begomovirus and confirmed by the BLAST program showed that papaya samples were infected with Ageratum yellow vein virus (AYVV). The phylogenetic results show that AYVV from papaya samples has a close relationship with the AYVV group from several other countries with 98% homology. Overall, our study further indicated Ageratum acts as an alternative host and reservoir for Begomovirus.
Publisher
Research Square Platform LLC
Reference38 articles.
1. Aidawati N, Hidayat SH, Suseno R, Sosromarsono S (2002) Transmission of an Indonesian isolat of Tobacco leaf curl virus (Geminivirus) by Bemisia tabaci Genn. (Hemiptera:Aleyrodidae). Plant Pathol. J 18(5): 231–236
2. Genetic diversity of Begomovirus infecting tomato and associated weed in Southeastern Brazil;Ambrozevicius LP;Fitopatol Bras,2002
3. Whitefly transmission of plant viruses;Brown JK;Adv Bot Res,2002
4. First report of Papaya leaf curl virus infecting papaya plants in Taiwan;Chang LS;Plant Dis,2003
5. Geminivirus classification and nomenclature: progress and problems;Fauquet CM;Ann Appl Biol,2003