Aflatoxin M1 binding by lactic acid bacteria in milk

Abstract

This research focused on biocontrol solution to increase food safety through studying lactic acid bacteria (LAB) which can bind aflatoxins in milk. Aflatoxins are toxic contaminants found in feeds and foods. In milk aflatoxin is found in metabolised form, aflatoxin M1 (AFM1). Three indigenous LAB Lactobacillus strains and one Lactococcus strain isolated from Kenyan spontaneously fermented foods were tested for their AFM1 binding abilities in different conditions and after different treatments along with two reference Lactobacillus strains. Binding of AFM1 in different concentrations was examined with unconcentrated, concentrated, heat treated and concentrated heat-treated LAB cultures. Observed binding of AFM1 by LAB varied between 11 to 100%, being approximately at the level of 40% throughout the analysis sets. The results of this study suggest that the aflatoxin binding ability by LAB strain is not strongly strain specific and depends on many external and condition variables. Also, the methods used in determination of aflatoxin binding warrant critical evaluation.