Effects of deoxynivalenol exposure at peripuberty over testicles of rats: structural and functional alterations

Abstract

Deoxynivalenol (DON) is related to reduced reproductive performance in males and females in several species. Children and adolescents showed a high risk of exposure to DON, however, no study has evaluated reproductive effects of DON at puberty. The present study aimed to evaluate the effects of DON at peripuberty on the testicles of pubertal rats. To achieve this, 10 Wistar rats (28 days old) were fed for 28 days with a DON-contaminated diet (9.4 mg/kg) or a control diet. After the experimental period, rats (56 days old) were euthanised and the following evaluations were performed in the testicles: dynamics of spermatogenesis, tubular morphometry, number of Sertoli cells and Leydig cells, analysis of caspase-3 expression, and the index of cell proliferation using the nucleolus organising regions (NOR) method. Ingestion of DON-contaminated diet induced a significant reduction in the number of Sertoli and Leydig cells and the number of seminiferous tubules in stage XIV. A significant increase in the number of NORs in seminiferous tubules in stage I-VI was observed in animals receiving the DON diet. No significant difference was noted in tubular morphometry or caspase-3 expression. Taken together, our results unravelled that the peripubertal exposure to DON compromised the testicular structure of pubertal rats, changing the dynamics of spermatogenesis.