Apocynin attenuates patulin-induced cytotoxicity through reduction of oxidation stress and apoptosis in HEK293cells

Abstract

Patulin (PAT) is a natural mycotoxin that commonly contaminates fruits and their derivative products and has been proven to induce cytotoxicity and oxidative damage in renal cells. In the present study, we aimed to evaluate the effect of apocynin, a potent phenolic antioxidant isolated from plants, on PAT-induced cell injury in human embryonic kidney (HEK293) cells. Compared with 7.5 μM PAT treatment alone, 10 μM apocynin co-treatment elevated cell viability, alleviated lactate dehydrogenase release and reduced caspase activities. Furthermore, apocynin inhibited reactive oxygen species overproduction, re-established mitochondria membrane potential and elevated intracellular ATP content. In addition, the results showed that apocynin aggrandized reduced glutathione (GSH) content, reduced oxidized glutathione (GSSG) content, raised the GSH/GSSG ratio and elevated superoxide dismutase, catalase, glutathione reductase, and glutathione peroxidase activities. Collectively, results of the study clearly show that apocynin supplement may serve as an alternative intervention to protect HEK293 cells against cytotoxicity induced by PAT through reduction of oxidation stress and apoptosis.