The Determination of Presence of Listeria monocytogenes in Ground Meat Sold in Istanbul
Author:
ULUDAĞ Arda Alara1, ARSLAN AYDOĞDU Elif Özlem2, KİMİRAN Ayten2
Affiliation:
1. İSTANBUL ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ, BİYOLOJİ (DR) 2. ISTANBUL UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY, DEPARTMENT OF FUNDAMENTAL AND INDUSTRIAL MICROBIOLOGY
Abstract
Among the 21 different species of the Listeria genus, Listeria monocytogenes is the most common and listeriosis agent in humans. The mortality rate of L. monocytogenes infection is higher than the other common foodborne pathogens such as Salmonella Enteritidis, Campylobacter and Vibrio species. In the current study, it is aimed to determine the presence of L. monocytogenes bacteria in minced meat samples sold in Istanbul province by using the culture method of the USDA-FSIS. In this study, 100 minced meat samples purchased from different butchers in 11 districts of Istanbul between December 2018 and November 2019 were examined for the presence of L. monocytogenes. The bacteria identified also molecularly verified according to the presence of iap and hlyA gene regions by the polymerase chain reaction (PCR) method. When antibiotic susceptibility tests of 21 strains, identified as L. monocytogenes through biochemical tests, it was found to be resistant to Amoxicillin/Clavulanic acid (14.28%), penicillin (9.52%), cefaclor (9.52%), vancomycin (9.52%), ciprofloxacin (9.52%) and trimethoprim-sulfamethoxazole (9.52%) and susceptible to ampicillin (100%) and tetracycline (100%). In terms of iap and hlyA gene regions, only 16 of 21 isolates, identified as L. monocytogenes as a result of biochemical tests, were found to be L. monocytogenes. In our study with minced meat offered for sale in Istanbul, although, the L. monocytogenes isolation rate (17%) and the incidence of antibiotic resistance of the isolated L. monocytogenes bacteria are low, it was concluded that minced meat may pose a public health risk.
Publisher
Gazi University Journal of Science
Subject
Multidisciplinary,General Engineering
Reference87 articles.
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