Evaluation of Antimicrobial Activity by Marine Nocardiopsis dassonvillei against Foodborne Listeria monocytogenes and Shiga Toxin-Producing Escherichia coli

Author:

Ngema Siyanda S.1,Khumalo Solomuzi H.1,Ojo Michael C.1ORCID,Pooe Ofentse J.2ORCID,Malilehe Tsolanku S.3,Basson Albertus K.1ORCID,Madoroba Evelyn1ORCID

Affiliation:

1. Department of Biochemistry and Microbiology, University of Zululand, Private Bag X1001, KwaDlangezwa 3886, South Africa

2. Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa

3. Department of Water and Sanitation, University of Limpopo, Private Bag X1106, Polokwane 0727, South Africa

Abstract

The emergence of multidrug-resistant pathogens creates public health challenges, prompting a continuous search for effective novel antimicrobials. This study aimed to isolate marine actinomycetes from South Africa, evaluate their in vitro antimicrobial activity against Listeria monocytogenes and Shiga toxin-producing Escherichia coli, and characterize their mechanisms of action. Marine actinomycetes were isolated and identified by 16S rRNA sequencing. Gas chromatography–mass spectrometry (GC–MS) was used to identify the chemical constituents of bioactive actinomycetes’ secondary metabolites. Antibacterial activity of the secondary metabolites was assessed by the broth microdilution method, and their mode of actions were predicted using computational docking. While five strains showed antibacterial activity during primary screening, only Nocardiopsis dassonvillei strain SOD(B)ST2SA2 exhibited activity during secondary screening for antibacterial activity. GC–MS identified five major bioactive compounds: 1-octadecene, diethyl phthalate, pentadecanoic acid, 6-octadecenoic acid, and trifluoroacetoxy hexadecane. SOD(B)ST2SA2′s extract demonstrated minimum inhibitory concentration and minimum bactericidal concentration, ranging from 0.78–25 mg/mL and 3.13 to > 25 mg/mL, respectively. Diethyl phthalate displayed the lowest bacterial protein-binding energies (kcal/mol): −7.2, dihydrofolate reductase; −6.0, DNA gyrase B; and −5.8, D-alanine:D-alanine ligase. Thus, marine N. dassonvillei SOD(B)ST2SA2 is a potentially good source of antibacterial compounds that can be used to control STEC and Listeria monocytogenes.

Funder

Red Meat Research and Development South Africa

Publisher

MDPI AG

Subject

Virology,Microbiology (medical),Microbiology

Reference90 articles.

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