The Present and Future Landscapes of Molecular Diagnostics

Author:

Rolando Justin C.123,Melkonian Arek V.123,Walt David R.123

Affiliation:

1. 1Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts, USA; email: dwalt@bwh.harvard.edu

2. 2Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA

3. 3Harvard Medical School, Harvard University, Boston, Massachusetts, USA

Abstract

Nucleic acid testing is the cornerstone of modern molecular diagnostics. This review describes the current status and future directions of molecular diagnostics, focusing on four major techniques: polymerase chain reaction (PCR), next-generation sequencing (NGS), isothermal amplification methods such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP), and clustered regularly interspaced short palindromic repeats (CRISPR)-based detection methods. We explore the advantages and limitations of each technique, describe how each overlaps with or complements other techniques, and examine current clinical offerings. This review provides a broad perspective into the landscape of molecular diagnostics and highlights potential future directions in this rapidly evolving field.

Publisher

Annual Reviews

Reference104 articles.

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