Author:
RAWAL REENA,KUMAR VINEET,RANI ANITA,GOKHALE S M,HUSAIN S M
Abstract
Lipoxygenase-2 is the prime contributor to the off- flavour associated with the soy products. With the aim to introgress null allele of lipoxygenase-2 into popular soybean variety JS 97-52 through marker assisted backcross selection (MABC), parental polymorphism was surveyed for JS 97- 52 (recipient parent) and PI 596540 (the donor of null allele of lipoxygenase-2). A total of 264 SSR markers dispersed across 20 linkage groups were screened. Of these, 130 SSR markers showed polymorphism. SSR markers Satt522 and Satt656 reported to be in the vicinity of Lox2 locus were found to be polymorphic for JS 97-52 and PI 596540 and were used for confirming true F1 and BC1F1 plants. The polymorphic SSR markers identified in the study would be utilized for retrieving the genetic background of JS 97-52 in the backcross generations.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
Agronomy and Crop Science
Reference13 articles.
1. Concibido V C, Valle B La, Mclaird P, Pineda N, Meyer J, Hummel L, Yand J, Delannay K Wu X. 2003. Introgression of a quantitative trait locus for yield from Glycine soja into commercial soybean cultivar. Theoretical and Applied Genetics 106: 575–82.
2. Davies C S and Nielsen N C. 1986. Genetic analysis of a null- allele for lipoxygenase-2 in soybean. Crop Science 26: 460–3.
3. Hildebrand D F and Hymowitz T. 1982. Inheritance of lipoxygenase-1 activity in soybean seeds. Crop Science 22: 851–3.
4. Kim M Y, Ha B K, Jun T H, Hwang, Kyunjung V, Kuk Y In and Lee S H. 2004. Single nucleotide polymorphism discovery and linkage mapping of lipoxygenase-2 gene. Euphytica 135: 169– 77.
5. Kim K H, Kim M Y, Van K, Moon J K, Kim D H and Lee S H. 2008. Marker Assisted foreground and background selection of near isogenic lines for bacterial leaf pustule resistant gene in soybean. Journal of Crop Science Technology 11(4): 263–8.