Author:
DHUTMAL RAJESH,MALOO SHIV RATAN,MORE A W,SHARMA VIJAY,ANU ,SINGH VIVEK K
Abstract
Considering the significance of sunflower (Helianthus annuus L.) cultivation under diverse agro-climatic conditions, present study was done to recognize assorted parental lines utilizing RAPD and ISSR markers to lessen environmental effects or test blunders at Rajasthan College of Agriculture, MPUA & T, Udaipur, Rajasthan during 2016. In the present study, RAPD primers produced 334 polymorphic band and ISSR primers produced 160 polymorphic bands. RAPD primers detected more polymorphic loci (56.88%) than the ISSR primers (52.24%). The dendrograms constructed by RAPD, ISSR primers and pooled data were associated with each other, indicating the efficacy of both marker systems in genetic diversity study of sunflower. Based on RAPD, ISSR and pooled data analysis, the restorer line EC 623023, R 16 and seed parental line CMS 234B, PET 2-7-1B were found to be genetically most diverse and fall into different groups. Therefore, their genetic origin seems to be different and could be efficiently used in sunflower breeding programs.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
Agronomy and Crop Science
Reference19 articles.
1. Behera T K, Gaikward A B, Singh A K and Staub J E. 2008. Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.). Journal of the Science of Food and Agriculture 88: 733–37.
2. DES. 2018. Agriculture statistics at a glance 2018. Directorate of Economics and Statistics, Department of Agriculture Cooperation and Farmer’s Welfare, Govt. of India.
3. Dudhe M Y, Ranganatha A R G and Vishnuvardhan Reddy A. 2019. Identification of restorers and maintainers from newly developed inbreds in sunflower. Bioscience Discovery 10(1): 21–24.
4. Galvan M Z, Bornet B, Balatti P A and Branchard M. 2003. Inter simple sequence repeat (ISSR) marker as a tool for the assessment of both genetic diversity and gene pool origin in common bean (Phaseolus vulgaris L.). Euphytica 132(3): 297–301.
5. Garayalde A F, Poverene M, Cantamutto M and Carrera A D. 2011. Wild sunflower diversity in Argentina revealed by ISSR and SSR markers: an approach for conservation and breeding programmes. Annals of Applied Biology 158(3): 305–17.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献