Assessment of free circulating DNA level and nucleotide profile at arterial hypertension-Reference-Cited by-同舟云学术

Assessment of free circulating DNA level and nucleotide profile at arterial hypertension

Published:2024-07-10 Issue:2 Volume:39 Page:160-165
ISSN:2713-265X
Container-title:Siberian Journal of Clinical and Experimental Medicine
language:
Short-container-title:SJCEM

Author:

Myachina O. V.¹^ORCID,Pashkov A. N.¹^ORCID,Kolesnikova E. V.²^ORCID,Korobova Y. R.¹^ORCID

Affiliation:

1. N.N. Burdenko Voronezh State Medical University of the Ministry of Healthcare of the Russian Federation

2. Voronezh City Hospital No. 20

Abstract

Arterial hypertension (AH) refers to socially significant diseases which cause a high level of primary disability and mortality. Identification of this pathology predictors, such as free circulating DNA (fc-DNA) level in blood, can provide early disease diagnostics, effective treatment, and, finally, a relatively favorable prognosis.Aim: To study fc-DNA level and nucleotide profile in blood plasm in patients with hypertension.Material and Methods. Study included 60 persons: 20 patients with stage II hypertension (group Hypertension II), 20 patients with stage III hypertension (group Hypertension III) and 20 healthy people (Control group). For fc-DNA isolation diaGene kit had been used. Obtained DNA samples were analyzed by liquid chromatography using FPLS® System (Sweden). Results. All samples (groups II, III and Control) involved not only fc-DNA, but also acid-soluble nucleotides. In patients with hypertension II and hypertension III fc-DNA content significantly increased compared to practically healthy individuals, regardless of disease stage (p < 0.05). The study also revealed a decrease in the level of adenosine and guanosine diphosphate (GDP) in the blood plasma in patients with hypertension, which was statistically significant in group III.Conclusion. The obtained data show new possibilities for cf-DNA and acid-soluble nucleotides using in blood plasma in patients with hypertension and determine further research of these indicators correlation with clinical features and prognosis of hypertension.

Publisher

Cardiology Research Institute

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