A simple antegrade perfusion method for isolating viable single cardiomyocytes from neonatal to aged mice

Author:

Omatsu-Kanbe Mariko1,Yoshioka Kengo1,Fukunaga Ryo1,Sagawa Hironori12,Matsuura Hiroshi1

Affiliation:

1. Department of Physiology; Shiga University of Medical Science; Otsu Shiga Japan

2. Department of Pediatrics; Shiga University of Medical Science; Otsu Shiga Japan

Funder

Japan Society for the Promotion of Science

Publisher

Wiley

Subject

Physiology (medical),Physiology

Reference20 articles.

1. A simplified, langendorff-free method for concomitant isolation of viable cardiac myocytes and nonmyocytes from the adult mouse heart;Ackers-Johnson;Circ. Res.,2016

2. Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse;Banerjee;Am. J. Physiol.,2007

3. Sodium current in single myocardial mouse cells;Benndorf;Pflügers Arch.,1985

4. Morphology and metabolism of intact muscle cells isolated from adult rat heart;Berry;Circ. Res.,1970

5. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches;Hamill;Pflügers Arch.,1981

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