CONTENT OF EXTRACTIVE SUBSTANCES AND POLYSACCHARIDES IN FRUIT BODIES OF GRIFOLA FRON-DOSA CULTIVATED ON LIGNOCELLULUS SUBSTRATES DEPENDING ON EXTRACTION METHODS

Abstract

The work is devoted to a comparative analysis of the quantitative content of extractives and water-soluble polysaccharides isolated from the fruit bodies of mushrooms Grifola frondosa depending on the extraction methods. As objects of study used strain of fungi G. frondosa 2639, isolated from commercial mycelium. It was established that the yield of fungi in polypropylene bags (volume up to 4500 cm3) was 36.37%, differing little from the yield of fruit bodies in glass containers (volume 800–1000 cm3). Using the hydrolysis of extraction products it was found that the methods of extracting the fruit bodies of G. frondosa with water lead to the extraction of polysaccharides of different composition. It was established that when using extraction methods in the Soxhlet apparatus, boiling and maceration (25.0±1.0 °C), the yield of extractive substances from the fruit bodies of G. frondosa was within the literature data interval and amounted to 6.04, 5.81 and 3.45%, respectively. It was shown that the highest content of water-soluble polysaccharides in the fruit bodies of G. frondosa was found using the extraction method in the Soxhlet apparatus – 14.92 %, differing from boiling and maceration methods (25.0±1.0 °C) in 1.15 and 1.33 times. By HPLC it was found that when maceration extraction was used (25.0±1.0 °C) and boiling, the monomeric composition of the polysaccharides of the fruit bodies of G. frondosa is mainly glucose, mannose, fucose and rhamnose in a ratio of 1.0 : 0.9 : 0.4 : 1.3, respectively. During extraction in the Soxhlet apparatus, their ratio was 1.0 : 1.2 : 0.5 : 1.9.