PREPARATION OF MEDICINAL PLANT RAW MATERIALS WITH A GIVEN CHEMICAL COMPOSITION AND ANTIBACTERIAL ACTIVITY

Abstract

A significant effect on the amount of hydroxycinnamic acids was not an increase in the content of bap from BA 1.0 to 10.0 μM, but the introduction of auxins of NAA 1.0 μM + IBA 0.1 μM. Auxins in combination with cytokinin BA increased the amount of acids by an average of 20% in alcoholic (1.9±0.2%) and aqueous (1.46±0.06%) extracts from Iris sibirica raw materials. The maximum yield of extractive substances, including tannins (4.4±0.3%) was observed when growing raw materials on media with a BA content of 2.5–5.0 μM, the lowest value was obtained with a BA of 10.0 μM. The introduction of auxins (NAA 1.0 μM + IBA 0.1 μM) did not significantly affect the dynamics of the accumulation of tannins. Screening of the chemical composition of medicinal plant raw materials Origanum vulgare and Salvia officinalis obtained by biotechnology methods showed the presence of flavonoids of 1.6±0.1% and 3.2±0.04%, hydroxycinnamic acids of 7.4% and 13.4% at a.s.s., respectively. Antibacterial activity with a minimum inhibitory concentration of 0.02-0.04 mg/ml against Sporosarcina ureae, Bacillus pumilus, Salmonella typhimurium, Staphylococcus aureus was pronounced in the hexane extract of Salvia officinalis. Ethanol extract of sage was active against Salmonella typhimurium. Ethanol extracts of Iris sibirica and Salvia officinalis showed antibacterial activity against Salmonella typhimurium, MIC-0.35 and 0.16 mg / ml, respectively. Siberian iris suppressed Bacillus pumilus at MIC-0.7 mg / ml. The largest amount of extractive substances was extracted from the raw materials of Origanum vulgare with an aqueous-glycerol mixture – 21.5±0.2 % per a. s. s. Against Salmonella typhimurium, the extracts showed antibiotic activity with MIC – 1.2 mg/ml.