Optimization of the cryoprotector composition for the preservation of African catfish reproductive cells-Reference-Cited by-同舟云学术

Optimization of the cryoprotector composition for the preservation of African catfish reproductive cells

Published:2023-10-13 Issue:3 Volume:2023 Page:47-56
ISSN:2073-5529
Container-title:Vestnik of Astrakhan State Technical University. Series: Fishing industry
language:en
Short-container-title:

Author:

Firsova Angelina Valerievna¹,Polovinkina Mariya Alexandrovna²,Osipova Victoria Pavlovna³,Ponomareva Elena Nikolaevna⁴⁵

Affiliation:

1. Astrakhan State Technical University; Federal Research Centre the Southern Scientific Centre of the Russian Academy of Sciences

2. Federal Research Centre the Southern Scientific Centre of the Russian Academy of Sciences

3. Federal Research Centre the Southern Scientific Centre of the Russian Academy of Sciences

4. Astrakhan State Technical University

5. Federal Research Centre The Southern Scientific Center of the Russian Academy of Sciences

Abstract

The article presents a study on the selection of the optimal composition of the protective medium for cryopres-ervation of the reproductive cells of male African catfish. Glycerol, dimethyl sulfoxide (DMSO) at concentrations of 3, 5 and 10% were studied as the main cryoprotective additives. The influence of these compounds on the quality indicators of catfish sperm (the percentage and time of sperm movement after activation), the level of sperm lipid peroxidation, and the activity of the antioxidant enzyme catalase before and after the cryopreservation process was assessed. A decrease in fish breeding indicators of the quality of African catfish sperm (the proportion of living cells, motility time) was established when DMSO was added to the basic cryomedium. The greatest decrease in the percentage of sperm motility before freezing is observed when DMSO is added at a concentration of 10%. A dose-dependent effect of a decrease in sperm activity after freezing was noted with an increase in the addition of DMSO to the cryogenic medium from 3 to 10%. It has been shown that 3% glycerol in a cryogenic medium does not contribute to the preservation of cells during freezing, cryodamage caused by the formation of ice crystals leads to a significant decrease in the motility of catfish sperm. The best indicators of sperm quality were obtained when using glycerol, the most effective concentration is 5% (40% of mobile reproductive cells within 53 s). The addition of 5% glycerol to the cryomedium contributes to the greatest decrease in the level of peroxidation of sperm lipids and an increase in catalase activity, which is fully consistent with the results on sperm motility. The calculated correlation coefficients confirm the dependence of the reproductive qualities of gametes on the antioxidant status of cells. The results indicate the importance of using antioxidants as cryoprotective additives to improve the quality of African catfish sperm breeding and indicate the need for more research to evaluate the effect of adding new potentially effective and safe antioxidants as cryoprotective additives.

Publisher

Astrakhan State Technical University

Subject

General Medicine

Reference25 articles.

1. Gjedrem T., Robinson N., Rye M. The importance of selective breeding in aquaculture to meet future demands for animal protein: A review // Aquaculture. 2012. V. 350–353. P. 117–129. https://doi.org/10.1016/j.aquaculture.2012.04.008., Gjedrem T., Robinson N., Rye M. The importance of selective breeding in aquaculture to meet future demands for animal protein: A review // Aquaculture. 2012. V. 350–353. P. 117–129. https://doi.org/10.1016/j.aquaculture.2012.04.008.

2. FAO. The State of World Fisheries and Aquaculture 2020. Sustainability in action. Rome: Food and Agriculture Organization of the United Nations, 2020. 224 р., FAO. The State of World Fisheries and Aquaculture 2020. Sustainability in action. Rome: Food and Agriculture Organization of the United Nations, 2020. 224 r.

3. Сидорова В., Асылбекова С., Январёва Н., Койшыбаева С., Бадрызлова Н., Мухрамова А., Шуткараев А. Экструдированные стартовые комбикорма для клариевого сома // Вестн. Астрахан. гос. техн. ун-та. Сер.: Рыбное хозяйство. 2020. № 2. С. 82–92. DOI: https://doi.org/10.24143/2073-5529-2020-2-82-93., Sidorova V., Asylbekova S., Yanvareva N., Koyshybaeva S., Badryzlova N., Muhramova A., Shutkaraev A. Ekstrudirovannye startovye kombikorma dlya klarievogo soma // Vestn. Astrahan. gos. tehn. un-ta. Ser.: Rybnoe hozyaystvo. 2020. № 2. S. 82–92. DOI: https://doi.org/10.24143/2073-5529-2020-2-82-93.

4. Nyang'ate Onura C., Van den Broeck W., Nevejan N., Muendo P., Van Stappen G. Growth performance and intestinal morphology of African catfish (Clarias gariepinus, Burchell, 1822) larvae fed on live and dry feeds // Aquaculture. 2018. V. 489. P. 70–79. https://doi.org/10.1016/j.aquaculture.2018.01.046., Nyang'ate Onura C., Van den Broeck W., Nevejan N., Muendo P., Van Stappen G. Growth performance and intestinal morphology of African catfish (Clarias gariepinus, Burchell, 1822) larvae fed on live and dry feeds // Aquaculture. 2018. V. 489. P. 70–79. https://doi.org/10.1016/j.aquaculture.2018.01.046.

5. Balogh R. E., Csorbai B., Guti C., Keszte S., Urbányi B., Orbán L., Kovács B. Validation of a male-specific DNA marker confirms XX/XY-type sex determination in several Hungarian strains of African catfish (Clarias gariepinus) // Theriogenology. 2023. V. 205. P. 106–113. https://doi.org/10.1016/j.theriogenology.2023.04.017., Balogh R. E., Csorbai B., Guti C., Keszte S., Urbányi B., Orbán L., Kovács B. Validation of a male-specific DNA marker confirms XX/XY-type sex determination in several Hungarian strains of African catfish (Clarias gariepinus) // Theriogenology. 2023. V. 205. P. 106–113. https://doi.org/10.1016/j.theriogenology.2023.04.017.