Production, Purification, and Characterization of a Potential Thermostable Galactosidase for Milk Lactose Hydrolysis from Bacillus stearothermophilus

Author:

Chen W.,Chen H.,Xia Y.,Zhao J.,Tian F.,Zhang H.

Funder

National Natural Science Foundation

National High Technology Research and Development Program

Publisher

American Dairy Science Association

Subject

Genetics,Animal Science and Zoology,Food Science

Reference23 articles.

1. Thermozymes and their applications: A review of recent literature and patents;Bruins;Appl. Biochem. Biotechnol.,2001

2. Studies on process engineering of galacto-oligosaccharides synthesis by beta-galactosidase from B. stearothermophilus;Chen,2001

3. Study on properties of thermostable β-galactosidase and hydrolysis of lactose in milk;Chen;China Dairy Ind.,2002

4. High-level expression of thermostable galactosidase gene in Escherichia coli;Chen;Biotechnology (N. Y.),2002

5. Cloning, expression, and purification of the His(6)-tagged thermostable beta-galactosidase from Pyrococcus woesei in Escherichia coli and some properties of the isolated enzyme;Daabrowski;Protein Expr. Purif.,2000

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