Cysticercus bovis in cattle slaughtered in North Egypt: Overestimation by the visual inspection method
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Published:2021
Issue:1
Volume:14
Page:155-160
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ISSN:2231-0916
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Container-title:January-2021
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language:en
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Short-container-title:Vet World
Author:
El-Sayad Mona Hassan1, Farag Hoda1, El-Taweel Hend1ORCID, Fadly Reda2, Salama Nahla3, Ahmed Asmaa Abd Elhameed4, Abd El-Latif Naglaa Fathi1ORCID
Affiliation:
1. Department of Parasitology, Medical Research Institute, Alexandria University, Egypt. 2. Department of Parasitology, Animal Health Research Institute, Agricultural Research Center, Egypt. 3. Department of Food and Science Technology, Faculty of Science, University of Alexandria, Egypt. 4. Department of Biomedical Informatics and Medical Statistics, Medical Research Institute, University of Alexandria, Egypt.
Abstract
Background and Aim: The World Health Organization and the Food and Agriculture Organization list Taenia saginata, a foodborne cestode, as the most widely distributed human tapeworm worldwide. The larval stage of T. saginata, Cysticercus bovis, causes cysticercosis in bovines and infects humans who eat raw or undercooked beef. The existing detection methods of C. bovis in cattle depend on the visual inspection of meat. This study aimed to confirm the identification of C. bovis through visual inspection at the slaughterhouses in North Egypt with a molecular diagnosis.
Materials and Methods: A total of 687 locally bred cattle (Baladi), including 428 cows and 259 buffaloes, slaughtered in four slaughterhouses in North Egypt from April 2018 to February 2019 were inspected for C. bovis using the traditional meat inspection method. Positive samples were verified through polymerase chain reaction (PCR) amplification and HDP2 gene sequencing.
Results: Through visual inspection, C. bovis was detected in 4.2% and 12.4% of the slaughtered cows and buffaloes, respectively. Molecular analysis confirmed that 1.9% of the animals, all of which were cows, had C. bovis infection. DNA sequencing verified the identity of the PCR-amplified product.
Conclusion: The rate of C. bovis infection in slaughterhouses detected through meat inspection is overestimated compared with that through PCR. Although meat inspection can be used as a primary screening tool for C. bovis, a more specific molecular method is required to achieve an accurate diagnosis.
Publisher
Veterinary World
Subject
General Veterinary
Reference24 articles.
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