Antibiograms, multidrug resistance, and milk-related parameters of bacteria isolated from milk of dairy cattle in Phatthalung, Thailand-Reference-Cited by-同舟云学术

Antibiograms, multidrug resistance, and milk-related parameters of bacteria isolated from milk of dairy cattle in Phatthalung, Thailand

Published:2024-03 Issue: Volume: Page:735-743
ISSN:2231-0916
Container-title:Veterinary World
language:en
Short-container-title:Vet World

Author:

Somrup Supaporn¹^ORCID,Mitsuwan Watcharapong²^ORCID,Bhumibhamon Teeraphun³,de Lourdes Pereira Maria⁴^ORCID,Paul Alok K.⁵^ORCID,Nissapatorn Veeranoot⁶^ORCID,Saengsawang Phirabhat⁷^ORCID

Affiliation:

1. Faculty of Technology and Community Development, Thaksin University, Phatthalung 93210, Thailand.

2. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand; Center of Excellence in Innovation of Essential Oils, Walailak University, Nakhon Si Thammarat 80160, Thailand.

3. Veterinary Research and Development Center (Upper Southern Region), Nakhon Si Thammarat 80110, Thailand.

4. Department of Medical Sciences, CICECO-Aveiro Institute of Materials, University of Aveiro, Aveiro 3810-193, Portugal.

5. School of Pharmacy and Pharmacology, University of Tasmania, Hobart, Australia.

6. School of Allied Health Sciences, Southeast Asia Water Team, World Union for Herbal Drug Discovery, and Research Excellence Center for Innovation and Health Products, Walailak University, Nakhon Si Thammarat 80160, Thailand.

7. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand.

Abstract

Background and Aim: Milk, a nutritious food, is widely consumed in human diets; however, contamination by micro-organisms can negatively impact its quality and consumer health. Contamination by micro-organisms affects the quality of milk, which can affect the quality of the milk production chain. This study aimed to determine the changes in milk composition and antibiotic susceptibility related to bacteria isolated from dairy cow milk. Materials and Methods: Raw milk samples were collected from 72 dairy cows. All milk samples were subjected to the California Mastitis Test (CMT) for CMT score determination. We also investigated milk composition, bacterial culture (BC), and antibiotic susceptibility. Results: About 47.22% and 30.56% of dairy cattle were positive for CMT + BC and automatic somatic cell count (ASCC) + BC, respectively. Fecal appearance and animal age were found to be risk factors for ASCC + BC positivity in dairy cattle. Bacteria were found in approximately 76% of milk samples, with the most common isolated species being hemolytic Streptococcus spp., coagulase-negative Staphylococcus spp., and Escherichia coli. Of these, 70% are resistant to at least one antibiotic. Variation in the multidrug resistance pattern was high in Klebsiella spp. Conclusion: Fecal appearance and animal age are risk factors for ASCC + BC positivity in dairy cattle. This study identified antibiotic and multidrug resistance patterns, which require comprehensive studies and effective surveillance systems. Remarkably, the use of antibiotic therapy in dairy cattle should be monitored. Keywords: antibiotic susceptibility, dairy cattle, milk composition, milk quality, Thailand.

Funder

Institute of Research and Development, Thaksin University

Centro de Investigação em Materiais Cerâmicos e Compósitos

Publisher

Veterinary World

Link

https://www.veterinaryworld.org/Vol.17/April-2024/1.pdf

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