Detection and molecular characterization of multiresistant Enterobacteriaceae carried by houseflies in the city of Bobo-Dioulasso, Burkina Faso-Reference-Cited by-同舟云学术

Detection and molecular characterization of multiresistant Enterobacteriaceae carried by houseflies in the city of Bobo-Dioulasso, Burkina Faso

Published:2024-01 Issue: Volume: Page:12-19
ISSN:2455-8931
Container-title:International Journal of One Health
language:en
Short-container-title:Int J One Health

Author:

Soufiane Sanou D. M.¹^ORCID,Serge Yerbanga R.²^ORCID,Tinlé Bangre³^ORCID,Sévérin N’Do⁴^ORCID,Firmin Kaboré N.¹^ORCID,Isidore Traoré¹^ORCID,Jacques Zoungrana⁵^ORCID,Inès Yaméogo³,Félicité Nikiéma³,Aminata Fofana⁴^ORCID,Naomi Batiéné³,Jean-Bosco Ouédraogo³^ORCID,Ibrahim Sangaré⁵^ORCID,Mahamoudou Sanou⁶^ORCID

Affiliation:

1. Centre MURAZ, Laboratoire de Biologie clinique, Bobo dioulaso, Burkina Faso.

2. Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso; Department of Microbiology, Institute of Research in Health Sciences, Western Regional Direction, Bobo Dioulasso, Burkina Faso.

3. Institut des Sciences et Technique (INSTech), Bacteriology Laboratory, Bobo-Dioulasso, Burkina Faso.

4. Department of Microbiology, Institute of Research in Health Sciences, Western Regional Direction, Bobo Dioulasso, Burkina Faso.

5. Department of Pharmacy, Nazi BONI University, INSSA, Bobo Dioulasso, Burkina Faso.

6. Department of Pharmacy, Joseph KI-ZERBO University, UFR/SDS, Ouagadougou, Burkina Faso.

Abstract

Background and Aim: Houseflies (Musca domestica) are synanthropic insects that are vectors of a wide range of multidrug-resistant pathogens responsible for infectious diseases. The aim of this study was to detect and characterize multidrug-resistant Enterobacteriaceae carried by houseflies in Bobo-Dioulasso. Materials and Methods: A total of 500 houseflies were captured in hospital and non-hospital environments in the city of Bobo-Dioulasso. For bacteriological analysis, they were divided into 125 batches of five flies each. Multidrug-resistant bacteria isolated on MacConkey agar supplemented with 4 μg/mL cefotaxime were identified on the basis of biochemical characteristics. Antibiotic susceptibility profiles were determined using the agar diffusion method. blaCTX-M resistance genes and quinolone resistance genes (plasmid-mediated quinolone resistance) were detected by conventional polymerase chain reaction. Results: Among 115 bacterial strains obtained, 26 were extended-spectrum beta-lactamase (ESBL)-producing enterobacteria: Escherichia coli (15), Klebsiella pneumoniae (6), Enterobacter cloacae (4), and Morganella morganii (1). Carriers were statistically more important in hospitals (12/26, p = 0.03). No carbapenem-resistance strains were observed. We identified ESBL resistance genes (Cefotaximase Munich; CTX-M group 1) (25/26) and quinolone resistance genes (QnrS) (6/26). Conclusion: Houseflies in the city of Bobo-Dioulasso are vectors for the transmission of multidrug-resistant enterobacteria. There is a need to monitor the associated risks for public health. Keywords: Bobo-Dioulasso, extended-spectrum beta-lactamase, Gram-negative bacilli, housefly, multiresistant Enterobacteriaceae.

Publisher

Veterinary World

Link

https://www.onehealthjournal.org/Vol.10/No.1/2.pdf

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