Estimation of upland rice samples for the presence of the drought resistance gene qDTY1.1 using a DNA marker

Abstract

Drought is the main stress factor when growing plants. The effects of drought on rice plants range from reduced dry matter accumulation to poor distribution of metabolites from stems and leaves into grain, resulting in reduced number of full grains per panicle, reduced grain weight and ultimately yield. Rice varietal diversity includes different drought tolerance genes. The purpose of the current research was to estimate upland rice samples and varieties for drought resistance under field conditions and the presence of the qDTY1.1 gene using the DNA marker RM431. PCR analysis was used to estimate 66 rice lines, hybrids and varieties grown in periodically wetted and constantly flooded fields. As a result of marker analysis, there has been identified the presence of the drought resistance gene qDTY1.1 in twenty-two rice varieties and samples such as ‘An-Yun-Ho’, ‘Volgogradsky’, ‘Stalingradsky’, ‘Volgogradsky x Atlant’, ‘Chan-Chun-Man’, etc. The highest value of the drought resistance index (DRI) was found in carriers of the qDTY1.1 allele, they are Manchurian upland varieties ‘An-Yun-Ho’ (79.4 %), ‘Chan-Chun-Man’ (88.5 %) and other samples developed earlier from crossing the variety ‘Chan-Chun-Man’ with productive varieties of Don selection ‘Razdolny’, ‘Boyarin’, ‘Komandor’, ‘Yuzhanin’, ‘Kuboyar’. The identified samples carrying the qDTY1.1 gene exceeded the other forms on average in terms of productivity under drought conditions by 0.20 t/ha, under the control by 0.21 t/ha, and under IZU by 3.9 %. In the long term of improving rice drought tolerance, it is necessary to identify and exploit other QTLs with large and consistent effects and key regulators of plant stress responses.